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KMID : 0545120180280111883
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 11 p.1883 ~ p.1895
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Comparison of Microbial Diversity and Composition in Jejunum and Colon of the Alcohol-dependent Rats
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Yang Fan
Zhao Ya-E
Wei Ji-Dong
Lu Yu-Fan
Zhang Ying
Sun Ya-Lun
Ma Meng-Yu
Zhang Rui-Ling
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Abstract
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Alcohol dependence was a global public health problem, yet the mechanisms of alcohol dependence has incompletely understood. The traditional view consider ethanol alter various neurotransmitters and their receptors in brain and develop the addiction. However, an increasing experimental evidences suggest that gut microbiota also influence brain functions via gut-to-brain interactions, and may therefore induce the development of alcohol use disorders. In this study, a rat model of alcohol dependence and withdrawal was employed, the gut microbiota composition was analyzed by high-throughput 16S rRNA gene sequencing, and the metagenome function was predicted by PICRUSt software. The results suggested that chronic alcohol consumption did not significant alter the diversity and richness of gut microbiota in jejunum and colon, but markedly changed the microbiota composition structure in colon. The phyla Bacteroidetes and eight genera including Bacteroidales S24-7, Ruminococcaceae, Parabacteroides, Butyricimonas, et al were drastically increased, however the genus Lactobacillus and gauvreauii were significantly decreased in alcohol dependence group compared with other two groups in the colon. The microbial functional prediction analysis revealed that the proportions of amino acid metabolism, polyketide sugar unit biosynthesis and peroxisome were significantly increased in AD group. This study demonstrated that the chronic alcohol consumption has a dramatic effect on the microbiota composition structure in the colon but few effects on the jejunum. Alcohol abuse induce the colonic microbiota dysbiosis seems to be a factor of alcohol dependence, which suggested that modulated the colonic microbiota composition might be a potentially new target for treating alcohol addiction.
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KEYWORD
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Alcohol dependence, gut microbiota, dysbiosis, jejunum, colon
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